This makes the approach attractive to situations and conditions with limited resources. It is also attractive for repeated testing and for moving estimated diagnostic resources to other parts of the skill chain. The examination through researchers from the Karolinska Institute published in the journal Nature Communications.
“We began to build on the factor of finding an easy-to-use test approach as soon as we saw advances in Asia and Southern Europe, and before the scenario reached a crisis point in Sweden,” says lead researcher Bjorn Reinius, head of studies in the Department of Medical Biochemistry and Biophysics at Karolinska Institutet. “Our approach was already over until the end of April, and then we made all the knowledge available online. “
The spread of the new coronavirus due in 2019 in China’s Wuhan region temporarily became a global pandemic. The maximum rate of transmission and a large number of asymptomatic infections have led to a massive global need for rapid, affordable and effective diagnostic tests that can only be carried out in clinical and non-clinical settings.
Diagnostic tests for COVID-19 are based on the detection of viral RNA in patient samples, such as nasal and throat samples, from which RNA molecules should be extracted and purified. RNA purification is a major bottleneck for the testing process, requiring a lot of appliances and logistics as well as expensive chemical compounds.
Simplifying existing strategies without particularly compromising their accuracy means more testing and faster, which would reduce transmission rates and facilitate early care.
The Karolinska Institutet Interdepartmental Research Group has developed strategies that go through the RNA extraction procedure so that once the patient’s pattern has been inactivated by warming, making the viral remains more infectious, it can go directly to the diagnostic reaction that detects the presence of the virus.
According to the researchers, the maximum keys to the good luck of the approach are the inactivation procedure of the previous virus and a new formula of the solution used to collect and send sampling devices taken from patients.
“By replacing the collection buffer with simple and affordable tampon formulations, we can enable viral detection with maximum sensitivity directly from the original clinical sample, without any intermediate steps,” says Dr. Reinius.
Institutions and study teams around the world have shown great interest in the approach since the publication of the first edition of the clinical paper on the medRxiv prepress server. The article has been read more than 15,000 times before even being reviewed by other researchers in the table and officially published in Nature Communications.
“Thanks to the low burden and simplicity of the method, this becomes a particularly exciting option in places and in conditions with limited resources, but an urgent desire to verify COVID-19,” he says, adding, “In fact, I would like to see that this control is also used in Sweden, for example, for regular and reasonable checks by other asymptomatic people in order to spread the infection. “
This story was published from a firm thread without converting the text. Only the name has been changed.
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